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Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos

dc.contributor.advisorCarazzone, Chiara
dc.contributor.authorMarin Mena, Karla Paola
dc.date.accessioned2022-07-11T20:32:23Z
dc.date.available2022-07-11T20:32:23Z
dc.date.issued2022-06-15
dc.identifier.urihttp://hdl.handle.net/1992/58727
dc.description.abstractLos carotenoides son pigmentos de estructura polienica con importantes funciones fotoprotectoras en las plantas, así como múltiples beneficios para la prevención y el tratamiento de enfermedades por sus propiedades antioxidantes, antinflamatorias y anticancerígenas en humanos. Por ello, en este trabajo de investigación se realizó la identificación putativa de carotenoides y derivados de clorofilas presentes ocho especies de ajíes que se consumen en diferentes regiones de Colombia, la cual se realizó mediante el análisis de sus espectros de absorción en la región UV-vis y los patrones de fragmentación obtenidos por espectrometría de masas. Empleando cromatografía líquida de alta resolución con detector de arreglo de diodos acoplada a un espectrómetro de masas en tándem (HPLC-DAD-APCI-MS/MS) se logró la identificación de 57 carotenoides libres y 72 carotenoides esterificados en las muestras analizadas. La separación cromatográfica se realizó empleando una columna C30 que posibilito diferenciar varios de los isómeros geométricos presentes en las muestras, como es el caso del 15-cis-, 13-cis-, 9-cis- y all trans-B-carotene, además de los isómeros 13-cis-B-Cryptoxanthin y all-trans-B-Cryptoxanthin. Adicionalmente, se realizó la identificación de otros carotenoides de tipo epóxidos como lo son: XX y YY, así como derivados de clorofilas. En conclusión, la metodología empleada fue idónea para la detallada caracterización de los carotenoides y derivados de clorofilas presentes en las ocho especies de ajíes.
dc.format.extent26 páginases_CO
dc.format.mimetypeapplication/pdfes_CO
dc.language.isospaes_CO
dc.publisherUniversidad de los Andeses_CO
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleIdentificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos
dc.typeTrabajo de grado - Pregradoes_CO
dc.publisher.programQuímicaes_CO
dc.subject.keywordCarotenoides
dc.subject.keywordAjí
dc.publisher.facultyFacultad de Cienciases_CO
dc.publisher.departmentDepartamento de Químicaes_CO
dc.contributor.juryZapata Rivera, Jhon Enrique
dc.contributor.juryMiscione, Gian Pietro
dc.contributor.juryHurtado Belalcazar, John Jady
dc.type.driverinfo:eu-repo/semantics/bachelorThesis
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.description.degreenameQuímicoes_CO
dc.description.degreelevelPregradoes_CO
dc.contributor.researchgroupLATNAPes_CO
dc.identifier.instnameinstname:Universidad de los Andeses_CO
dc.identifier.reponamereponame:Repositorio Institucional Sénecaes_CO
dc.identifier.repourlrepourl:https://repositorio.uniandes.edu.co/es_CO
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