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dc.contributor.advisorNarváez Noguera, Diana María
dc.contributor.advisorHernández Flórez, Luis Jorge
dc.contributor.authorBernal Morales, Johan Fabian
dc.descriptionExiste una brecha entre la generación del conocimiento en investigación y su aplicación en las prácticas, reconocida como un factor contributivo a las inequidades en salud, que puede ser abordada desde las prácticas basadas en la evidencia para la gestión del conocimiento y de las tecnologías en salud en Colombia.
dc.description.abstractThe evidence-based practice (EBP) in health has been described previously in health approaches. The technologies applied to health, such as whole genome sequencing (WGS), provide a better understanding of emerging risks as antimicrobial resistance in priority pathogens due to the massive data generation and the increased resolution in public health investigations. The information related to WGS implementation in low- and middle-income countries (LMICs) is scarce, and the cost and complexity have been described as challenges for the formal implementation. The strengthening of epidemiological surveillance and the incorporation of new technologies in the public health system are described in the health plans in Colombia. Therefore, this study aims to provide an EBP for the WGS implementation in pathogen laboratory surveillance at a national reference laboratory in Colombia. To accomplish this goal, a concept model resulted from the integration of implementing science elements contained in the Iowa model, the PRACTIS step-by-step framework and the end-user behaviors, the review of information, local research implementation, and previous experiences in WGS, were used to build a structured EBP as a theoretical proposal. The results evidenced a positive perception of the commitment in strategic stakeholders for future implementation of WGS as routine in pathogen surveillance in Colombia. The resulting structure of the EBP was built on strata, distributed in action levels, categories and parameters. The theoretical EBP was recognized, criticized and evaluated by groups of people from two national reference laboratories, in two different roles, as developers and as end-users. An advisor groups of experts were involved looking for the addressing of local barriers and recommendations. The EBP proposed in this study improved the knowledge and understanding of all the strategic audiences of the national action plan on WGS implementation requirements in a local context in Colombia. The EBP for WGS could be set as the first step to the formal implementation planning in a national reference laboratory in Colombia due to the synthesis and intuitive structure for all audiences. Additionally, the EBP also promotes quick and strategic driven WGS for all audiences on novel health initiatives locally. The EBP in LMICs could be an essential resource on health knowledge translation in local action and policies, overcoming access barriers and promoting the evidence-based decisions in health.
dc.format.extent24 páginases_CO
dc.publisherUniversidad de los Andeses_CO
dc.titleAn evidence-based practice for the implementation of whole genome sequencing at a national reference laboratory in Colombia
dc.title.alternativeUna práctica basada en la evidencia para la implementación de secuenciación de genoma completo en un laboratorio nacional de referencia en Colombia
dc.typeTrabajo de grado - Maestríaes_CO
dc.publisher.programMaestría en Salud Públicaes_CO
dc.subject.keywordAntimicrobial resistance
dc.subject.keywordWhole genome sequence
dc.subject.keywordLow- and middle-income countries
dc.subject.keywordEvidence-based practice
dc.publisher.facultyEscuela de Gobierno Alberto Lleras Camargoes_CO
dc.contributor.juryPrieto Alvarado, Franklyn Edwin
dc.contributor.juryZapata Bedoya, Silvana
dc.description.degreenameMagíster en Salud Públicaes_CO
dc.contributor.researchgroupInvestigación y Vigilancia Integrada de la Resistencia Antimicrobianaes_CO
dc.description.researchareaLínea de investigación recolección de especímeneses_CO
dc.description.researchareaMonitoreo de Resistencia antimicrobiana en bacterias de origen animales_CO
dc.identifier.instnameinstname:Universidad de los Andeses_CO
dc.identifier.reponamereponame:Repositorio Institucional Sénecaes_CO
dc.relation.referencesMurray CJ, Shunji Ikuta K, Sharara F, Swetschinski L, Robles Aguilar G, Gray A, et al. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. The Lancet [Internet]. 2022 [cited 2022 Sep 22];399:629-55. Available from:
dc.relation.referencesDadgostar P. Antimicrobial Resistance: Implications and Costs. 2019; Available from:
dc.relation.referencesWHO. Global Action Plan on Antimicrobial Resistance. Microbe Magazine. 2015;10(9):354-5.es_CO
dc.relation.referencesColombia M de S y protección social de. Plan nacional contra la resistencia a los antimicrobianos. Ministerio de Salud y protección social de Colombia 2018 p. 43.es_CO
dc.relation.referencesTekola-Ayele F, Rotimi CN. Translational Genomics in Low and Middle Income Countries: Opportunities and Challenges. Public Health Genomics. 2015 Jul 25;18(4):242.es_CO
dc.relation.referencesOkeke IN, Ihekweazu C. The importance of molecular diagnostics for infectious diseases in low-resource settings. Nature Reviews Microbiology 2021 19:9 [Internet]. 2021 Jun 28 [cited 2022 Feb 21];19(9):547-8. Available from:
dc.relation.referencesOrganización panamericana de salud (OPS). Red Regional de Vigilancia Genómica de COVID-19. 2022.es_CO
dc.relation.referencesLaiton-Donato K, Franco-Muñoz C, Álvarez-Díaz DA, Ruiz-Moreno HA, Usme-Ciro JA, Prada DA, et al. Characterization of the emerging B.1.621 variant of interest of SARS-CoV-2. Infection, Genetics and Evolution. 2021 Nov 1;95.es_CO
dc.relation.referencesRamirez AL, Luna N, Patiño LH, Castañeda S, Muñoz M, Ballesteros N, et al. Impact of SARS-CoV-2 Mu variant on vaccine effectiveness: A comparative genomics study at the peak of the third wave in Bogota, Colombia. J Med Virol. 2022 Aug 1;94(8):3988-91.es_CO
dc.relation.referencesSecretaría Distrital de Salud de Bogotá D. C. Plan Territorial de Salud para Bogotá, D. C. 2016-2020. Alcaldía Mayor de Bogotá, D. C. Secretaría Distrital de Salud de Bogotá D. C. 2016. 283 p.es_CO
dc.relation.referencesRuíz F, Escobar GM, Milena Cuellar Segura C, Teresa Buitrago Grupo ME, María Henríquez Grupo GM, Stella Ríos Grupo LM, et al. PLAN DECENAL DE SALUD PÚBLICA PDSP 2022 - 2031. Bogota; 2022.es_CO
dc.relation.referencesCongreso de Colombia. LEY ESTATUTARIA No. 1751. Bogota; 2015.es_CO
dc.relation.referencesKhoury MJ, Bowen MS, Clyne M, Dotson WD, Gwinn ML, Green RF, et al. From public health genomics to precision public health: a 20-year journey. Genetics in Medicine 2018 20:6. 2017 Dec 14;20(6):574-82.es_CO
dc.relation.referencesTimme RE, Rand H, Leon MS, Hoffmann M, Strain E, Allard M, et al. GenomeTrakr proficiency testing for foodborne pathogen surveillance: An exercise from 2015. Microb Genom [Internet]. 2018 Jul 1 [cited 2021 Jul 26];4(7). Available from:
dc.relation.referencesBentley SD, Lo SW. Global genomic pathogen surveillance to inform vaccine strategies: a decade-long expedition in pneumococcal genomics. Genome Medicine 2021 13:1. 2021 May 17;13(1):1-12.es_CO
dc.relation.referencesVegyari C, Underwood A, Kekre M, Argimon S, Muddyman D, Abrudan M, et al. Whole-genome sequencing as part of national and international surveillance programmes for antimicrobial resistance: A roadmap [Internet]. Vol. 5, BMJ Global Health. BMJ Specialist Journals; 2020 [cited 2021 Jul 26]. p. e002244. Available from:
dc.relation.referencesJylhä V, Oikarainen A, Perälä ML, Holopainen A. Facilitating evidence-based practice in nursing and midwifery in the WHO European Region [Internet]. 2017. Available from:
dc.relation.referencesNilsen P. Making sense of implementation theories, models and frameworks. Implementation Science 2015 10:1. 2015 Apr 21;10(1):1-13.es_CO
dc.relation.referencesFerdinand AS, Kelaher M, Lane CR, da Silva AG, Sherry NL, Ballard SA, et al. An implementation science approach to evaluating pathogen whole genome sequencing in public health. Genome Medicine 2021 13:1 [Internet]. 2021 Jul 28 [cited 2021 Sep 19];13(1):1-11. Available from:
dc.relation.referencesMaljkovic Berry I, Melendrez MC, Bishop-Lilly KA, Rutvisuttinunt W, Pollett S, Talundzic E, et al. Next Generation Sequencing and Bioinformatics Methodologies for Infectious Disease Research and Public Health: Approaches, Applications, and Considerations for Development of Laboratory Capacity. J Infect Dis [Internet]. 2020 Mar 28 [cited 2021 Aug 5];221(Supplement_3):S292-307. Available from:
dc.relation.referencesCullen L, Hanrahan K, Edmonds SW, Reisinger HS, Wagner M. Iowa Implementation for Sustainability Framework. Implementation Science. 2022 Dec 1;17(1).es_CO
dc.relation.referencesKlein HJ, Cooper JT, Molloy JC, Swanson JA. The assessment of commitment: Advantages of a unidimensional, target-free approach. Journal of Applied Psychology. 2014;99(2):222-38.es_CO
dc.relation.referencesTorraco RJ. Writing Integrative Reviews of the Literature. International Journal of Adult Vocational Education and Technology. 2016 Jul 1;7(3):62-70.es_CO
dc.relation.referencesKoorts H, Eakin E, Estabrooks P, Timperio A, Salmon J, Bauman A. Implementation and scale up of population physical activity interventions for clinical and community settings: the PRACTIS guide. International Journal of Behavioral Nutrition and Physical Activity 2018 15:1 [Internet]. 2018 Jun 8 [cited 2021 Sep 22];15(1):1-11. Available from:
dc.relation.referencesGlasgow RE, Estabrooks PE. Peer Reviewed: Pragmatic Applications of RE-AIM for Health Care Initiatives in Community and Clinical Settings. Prev Chronic Dis. 2018;15(1):170271.es_CO
dc.relation.referencesIowa Model Collaborative. Iowa Model of Evidence-Based Practice: Revisions and Validation Authored on behalf of the Iowa Model Collaborative [Internet]. Iowa; 2017 [cited 2022 Oct 29]. Available from:
dc.relation.referencesChiwaula CH, Kanjakaya P, Chipeta D, Chikatipwa A, Kalimbuka T, Zyambo L, et al. Introducing evidence based practice in nursing care delivery, utilizing the Iowa model in intensive care unit at Kamuzu Central Hospital, Malawi. Int J Afr Nurs Sci. 2021 Jan 1;14.es_CO
dc.relation.referencesDonado-Godoy P, Bernal JF, Rodríguez F, Gomez Y, Agarwala R, Landsman D, et al. Genome Sequences of Multidrug-Resistant Salmonella enterica Serovar Paratyphi B (dT+) and Heidelberg Strains from the Colombian Poultry Chain. Genome Announc [Internet]. 2015 [cited 2022 Jan 31];3(5). Available from:
dc.relation.referencesBernal JF, Donado-Godoy P, Arévalo A, Duarte C, Realpe ME, Díaz PL, et al. Whole-genome sequence of multidrug-resistant campylobacter coli strain COL B1-266, isolated from the colombian poultry Chain. Genome Announc. 2016;4(2).es_CO
dc.relation.referencesNadon C, van Walle I, Gerner-Smidt P, Campos J, Chinen I, Concepcion-Acevedo J, et al. PulseNet International: Vision for the implementation of whole genome sequencing (WGS) for global food-borne disease surveillance. Euro Surveill [Internet]. 2017 Jun 8 [cited 2022 Jan 31];22(23). Available from:
dc.relation.referencesHendriksen RS, Pedersen SK, Leekitcharoenphon P, Malorny B, Borowiak M, Battisti A, et al. Final report of ENGAGE - Establishing Next Generation sequencing Ability for Genomic analysis in Europe. EFSA Supporting Publications [Internet]. 2018 Jun 1 [cited 2022 Jan 31];15(6):1431E. Available from:
dc.relation.referencesGerner-Smidt P, Hendrik Havelaar A, Franz E, Jean Struelens M, Besser J, Concepción-Acevedo J, et al. Whole Genome Sequencing: Bridging One-Health Surveillance of Foodborne Diseases. Front Public Health [Internet]. 2019;7(172):172. Available from: https://www.es_CO
dc.relation.referencesS ND, V S. Evidence Based Practice- A Review. Saudi Journal of Nursing and Health Care. 2022 May 20;5(5):105-6.es_CO
dc.relation.referencesIoannidis JPA, Khoury MJ. Evidence-based medicine and big genomic data. Vol. 27, Human Molecular Genetics. Oxford University Press; 2018. p. R2-7.es_CO
dc.rights.licenceAtribución 4.0 Internacional*
dc.subject.themesGobierno y Asuntos Públicoses_CO

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